In vitro study | NFAT Inhibitor treatment significantly inhibits nuclear translocation of NFATc1 for 24 hours. Long-term treatment with VIVIT significantly inhibits the cytoplasmic levels of cathepsin K, TRAP, and MMP-9. Western Blot Analysis Cell Line: Human peripheral blood CD14 + monocytes Concentration: 10 μM Incubation Time: 24 hours or 21 days Result: Short-term treatment with 10 μM, significantly inhibited nuclear translocation of NFATc1. Long-term treatment, significantly inhibited the cytoplasmic levels of cathepsin K, TRAP, and MMP-9. |